Overexpression of Penicillin V Acylase from Streptomyces lavendulae and 1 Elucidation of its Catalytic Residues
نویسندگان
چکیده
a Department of Biochemistry and Molecular Biology I, Faculty of Biology, University 7 Complutense of Madrid, Madrid, Spain; and b Department of Environmental Biology, Centro 8 de Investigaciones Biológicas, CSIC, Madrid, Spain 9 10 Running head: Approach to catalytic mechanism of Penicillin V acylase from Streptomyces 11 lavendulae 12 #Address correspondence to Isabel de la Mata, [email protected] 13
منابع مشابه
Overexpression of penicillin V acylase from Streptomyces lavendulae and elucidation of its catalytic residues.
The pva gene from Streptomyces lavendulae ATCC 13664, encoding a novel penicillin V acylase (SlPVA), has been isolated and characterized. The gene encodes an inactive precursor protein containing a secretion signal peptide that is activated by two internal autoproteolytic cleavages that release a 25-amino-acid linker peptide and two large domains of 18.79 kDa (alpha-subunit) and 60.09 kDA (beta...
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